Fievel was voiced by Phillip Glasser, who later did voicework for A Troll in Central Park. The voices of Mama and Papa are Erica Yohn and Nehemiah Persoff, who had no other roles in animation. Dom DeLuise, who also voiced Don Bluth's The Secret of NIMH, All Dogs Go to Heaven, and A Troll in Central Park, was the voice of Tiger, a fat but friendly vegetarian cat that Fievel befriends (who presumably represents the less prejudiced of American citizens). In the simultaneously-released Latin Spanish version of the film, Fievel was voiced by Laura Bustamante.
Note that while all of the animal characters were animated from scratch, the human characters are portrayed using the rotoscoping technique, in which sequences were shot in live action and then traced onto animation cels. This provides a realistic look for human characters, and distinguishes the cartoonish animal characters from the more realistically-animated humans. Rotoscoping is frequently employed in Don Bluth films, including The Secret of NIMH and Anastasia.
The film was released on VHS in the same year by CIC Video and is now available on a DVD that contains the main English track, as well as dubbing for French and Spanish.